Assessment Items from the Molecular Biology 2 Small Group

Learning Objective: Describe the relationship between a gene and the product it encodes.

Formative Assessment
Given a 100 amino acid protein, how many nucleotides would its gene have?
A) 100
B) 200
C) 300
D) more than 300

Summative Assessment #1
Imagine you are interested in expressing a human gene in E. coli on a plasmid vector. The gene contains 3 exons and 2 introns. Check all of the items that you will need to have on the plasmid in order to accomplish this task (2 items have been checked for you).

Human

E.coli

Selectable Marker
X
Selectable Marker

Origin of Replication
X
Origin of Replication

Ribosome Binding Site

Ribosome Binding Site

Promoter

Promoter

Exon 1

Exon 1

Exon 2

Exon 2

Exon 3

Exon 3

Intron 1

Intron 1

Intron 2

Intron 2

transcriptional terminator

transcriptional terminator

Summative Assessment #2
Is it possible for the same gene to encode different proteins in different cells in humans? True or False

Why did you choose your answer above? (open-ended)